Introduction to NGS Data Analysis

This Introduction to NGS Data Analysis is a two-day, hands-on workshop that offers attendees a basic understanding of NGS data analysis workflows. The workshop provides hands-on computational experience in analysis of NGS data using common analytical approaches for ChIP-Seq and RNA-Seq data.

Workshop Outline

Topics covered by this workshop include:

  • An introduction to the command line interface and NGS file formats
  • Assessment of the quality of NGS sequence reads
  • Sequence alignment algorithms
  • Basic ChIP-Seq analysis
  • Basic RNA-Seq analysis

Workshop Dates

City Location Date Workshop Costs
Melbourne Monash University 14th June – 15th June 2016 $330 (Plus GST)

Recommended Participants

The Introduction to NGS Data Analysis workshop is designed for bench scientists, PhD students and early career postdoctoral researchers who require NGS in their research area but have minimal bioinformatics experience. The workshop utilises the Linux command-line, although no prior programming experience is required.

Workshop Coordinators

Katherine Champ, Bioplatforms Australia (

Draft Program

Day 1 – Introduction to the command line, data quality & alignment & ChIP-Seq



09:00 – 09:45 Introductions and course orientation
09:45 – 10:15 Practical: Introduction to the command line course
10:15 – 10:40 Morning Tea
10:40 – 11:20 Practical: Introduction to the command line course (cont.)
11:20 – 11:45 Introduction to NGS Course
11:45 – 12:30 Introduction to NGS – Technology, data formats and quality control
12:30 – 13:15 Lunch
13:15 – 13:25 Quality control intro to practical
13:25 – 14:05 Practical: Quality Control
14:05 – 14.15 Introduction to sequence alignment
14:15 – 15:00 Practical: Sequence Alignment
15:00 – 15:25 Afternoon Tea
15:25 – 15:55 Introduction to ChIP-Seq
15:55 – 17:30 Practical: ChIP-Seq analysis – Peak calling and annotation

Day 2 – ChIP-Seq motif analysis and RNA-Seq analysis



09:00 – 09:40 Practical: Motif analysis
09:30 – 10:30 Introduction to RNA-Seq
10:30 – 10:50 Morning Tea
10:50 – 12:30 Practical: Alignment and splice junction identification
12:30 – 13:30 Lunch
13:30 – 15:00 Practical: Transcriptome assembly
15:00 – 15:30 Afternoon Tea
15:30 – 17:30 Practical: Differential gene expression

Registration and Payment

Workshop places are limited due to the significant practical component of this workshop. To manage the high demand, all workshop applicants are subject to a selection process and MUST complete the online application form to be considered for a place. Incomplete applications will not be considered. The application period closes 10th May 2016. Successful applicants will be notified no later than 16th May 2016. The workshop fee of $330.00 (plus GST) covers course material, computer access, lunch and refreshments. Payment can be made by cheque, EFT or credit card.


For further information contact Katherine Champ,


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