RNA-Seq Analysis Using Galaxy

RNA-Seq Analysis Using Galaxy is a one-day, workshop that introduces the concepts of RNA-Seq analysis. This workshop provides hands-on experience with RNA-Seq data preparation, statistical testing for differential gene expression, identifying novel transcription features and generating graphical summaries of RNA-Seq data.

The workshop will focus on the use of Galaxy, a platform that provides a simple and user-friendly interface to bioinformatics tools, and will also feature an introduction to the R environment used widely by bioinformaticians.

Course Outline

During this course you will learn about;

    • The tools and workflows of RNA-Seq analysis
    • Analysis of RNA-Seq data for organisms with reference genomes
    • Web-based and command-line interfaces to bioinformatics tools

Recommended Participants

RNA-Seq analysis using Galaxy is designed for bench scientists, PhD students and early career postdoctoral researchers who are planning to work with next-generation sequencing data for small variant detection. No prior bioinformatics experience is required.

This workshop is open to all Australian life scientists, however priority will be given to NSW and ACT, health and medical researchers.

The Trainers

  • Dr Mark Crowe

Workshop Dates

City Location Date Cost
Newcastle University of Newcastle 3rd December 2015 $165.00 incl. GST

Draft Program

Time Topic
09:00 Introductions and workshop orientation
Principles of RNA-Seq analysis
How to access Galaxy
Quality control of NGS data
10:15 Morning Tea
10:45 Read mapping
Visualising mapped data using genome browsers
Statistical analysis of mapped reads
12:30 Lunch
13:30 Identification of novel transcription features
Self-guided exercise – a DGE workflow
EdgeR – alternative software for RNA-Seq analysis
15:15 Afternoon Tea
15:45 EdgeR continue
Gene list enrichment analysis
Other Galaxy features – uploading data, histories, workflows
17:20 Final wrap-up and close

Registration and Payment

Due to the practical component of this workshop, places are strictly limited to 25 participants. To manage the high demand, all workshop applicants are subject to a selection process and MUST complete the online application form to be considered for a place. Incomplete applications will not be considered.

The workshop fee of $165 (including GST) covers course material, computer access, lunch and refreshments. Payment can be made by cheque, EFT or credit card.

The application period closes 16th November 2015. Successful applicants will be notified no later than 20th November 2015.

If you are unable to apply online please download the application form and email the completed form to kchamp@bioplatforms.com.

For further information contact Katherine Champ, kchamp@bioplatforms.com.

Workshop registration

Competition for places in these workshops is high. All applications will be assessed via a selection process.

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Interested in Hosting a Bioinformatics Workshop?

If you are interested in hosting a bioinformatics workshop at your local organisation or institute please contact Katherine Champ on kchamp@bioplatforms.com.